8HHG

The bacterial divisome protein complex FtsB-FtsL-FtsQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-28
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	I 1 2 1
Unit cell lengths	101.090, 53.850, 177.418
Unit cell angles	90.00, 93.93, 90.00

Refinement procedure

Resolution	34.452 - 3.100
R-factor	0.2448
Rwork	0.243
R-free	0.28580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8hhf
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	88.500	88.500	3.310
High resolution limit [Å]	3.100	8.770	3.100
Rmerge	0.170	0.068	1.232
Total number of observations	131166	5282	23767
Number of reflections	17628	807	3161
<I/σ(I)>	6.9	16.2	1.6
Completeness [%]	99.8	96.1	100
Redundancy	7.4	6.5	7.5
CC(1/2)	0.996	0.996	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1M Tris pH 8.0, 20-35% PEG400