8HHF

The bacterial divisome protein complex FtsB-FtsL-FtsQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	199.610, 53.940, 101.850
Unit cell angles	90.00, 116.67, 90.00

Refinement procedure

Resolution	49.850 - 3.040
R-factor	0.2712
Rwork	0.270
R-free	0.29390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z2w
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.850	49.850	2.980
High resolution limit [Å]	2.900	12.970	2.900
Rmerge	0.126	0.066	3.192
Rmeas	0.133	0.070	3.348
Total number of observations	219340
Number of reflections	21882	272	1620
<I/σ(I)>	9.25	24.4	0.84
Completeness [%]	99.7	97.1	99.6
Redundancy	10.024	8.993	10.369
CC(1/2)	0.998	0.997	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1M Tris ph 8.0, 20-35% PEG400