8HHE
Crystal structure of Cry5B from Bacillus thuringiensis at 4.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99984 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 114.401, 114.401, 263.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 4.500 |
| R-factor | 0.2405 |
| Rwork | 0.238 |
| R-free | 0.28850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d8m |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18_3861) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.740 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.274 | 0.559 |
| Number of reflections | 10954 | 1560 |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 305 | 2500 mM NaCl, 100 mM sodium cacodylate pH 6.5, and 200 mM Li2SO4 |
