8HHB
F1 domain of FoF1-ATPase from Bacillus PS3,step waiting,lowATP
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.3 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.5 |
| Number of Particle | 2654860 |
| Software | RELION,cryoSPARC,UCSF ChimeraX,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
