8HGU
Epoxide hydrolase from Bosea sp. PAMC 26642
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-04-27 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.537, 84.913, 98.235 |
| Unit cell angles | 90.00, 100.91, 90.00 |
Refinement procedure
| Resolution | 30.200 - 1.940 |
| R-factor | 0.1739 |
| Rwork | 0.172 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ehy |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.748 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.940 | 1.950 |
| Rmerge | 0.110 | 0.410 |
| Number of reflections | 101095 | 4384 |
| <I/σ(I)> | 29.96 | 4.23 |
| Completeness [%] | 97.6 | 85.3 |
| Redundancy | 4.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296.15 | 1 M Ammonium formate, 0.1 M Sodium acetate pH 5.0, 8 % w/v PGA (Na+ form, LM) |
