8HGQ
The apo-flavodoxin dimer from Synechococcus elongatus PCC 7942
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-09 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 49.560, 49.560, 161.025 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.000 - 2.090 |
| R-factor | 0.1941 |
| Rwork | 0.192 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1czn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.764 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.087 | 0.483 |
| Rpim | 0.022 | 0.189 |
| Number of reflections | 12295 | 1772 |
| <I/σ(I)> | 20 | 2.9 |
| Completeness [%] | 96.6 | 98.8 |
| Redundancy | 16.2 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.4 M NaH2PO4/1.6 M K2HPO4; 0.1 M imidazole PH8.0; 0.2 M NaCl |
