8HEW
Potato 14-3-3 St14f
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-02-21 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 121.628, 121.628, 69.648 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.590 |
| Rwork | 0.243 |
| R-free | 0.26530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o9c |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.630 |
| High resolution limit [Å] | 2.590 | 7.020 | 2.590 |
| Rmerge | 0.103 | 0.036 | 0.561 |
| Rmeas | 0.111 | 0.039 | 0.615 |
| Rpim | 0.041 | 0.015 | 0.245 |
| Total number of observations | 131010 | ||
| Number of reflections | 18669 | 976 | 887 |
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 99.5 | 96.8 | 96.4 |
| Redundancy | 7 | 6.8 | 5.9 |
| CC(1/2) | 0.999 | 0.867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 12% (w/ v) polyethylene glycol (PEG) 8000, 0.2M ammonium tartrate, pH 7 |
