8HDL
Crystal structure of ASFV trans geranylgeranyl diphosphate synthase B318L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 56.130, 56.130, 376.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.210 - 3.198 |
| R-factor | 0.2497 |
| Rwork | 0.246 |
| R-free | 0.28380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.421 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.210 | 3.280 |
| High resolution limit [Å] | 3.198 | 3.200 |
| Rmerge | 0.297 | |
| Number of reflections | 6579 | 472 |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 99.7 | |
| Redundancy | 31.7 | |
| CC(1/2) | 0.998 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M HEPES pH 7.5, 4.3 M NaCl |
