8HD5

The crystal structure of Hu protein in Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-18
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	41.427, 82.369, 61.633
Unit cell angles	90.00, 93.16, 90.00

Refinement procedure

Resolution	29.190 - 2.140
R-factor	0.2002
Rwork	0.197
R-free	0.26240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qjn
RMSD bond length	0.008
RMSD bond angle	0.875
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.190	2.220
High resolution limit [Å]	2.140	2.140
Number of reflections	22347	2190
<I/σ(I)>	12.62
Completeness [%]	98.0
Redundancy	2
CC(1/2)	0.998	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	296	0.1M MES, 15% PEG 3350