8HD2
Crystal structure of SAM dependent methyltransferase encoded in type II fatty acid biosynthesis gene cluster from ladderane lipid producing anammox bacteria
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 35.016, 48.060, 49.828 |
Unit cell angles | 82.59, 84.12, 70.67 |
Refinement procedure
Resolution | 45.120 - 1.600 |
R-factor | 0.1814 |
Rwork | 0.180 |
R-free | 0.20780 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.852 |
Data reduction software | DIALS |
Data scaling software | pointless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.120 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 38897 | 1761 |
<I/σ(I)> | 7 | |
Completeness [%] | 97.1 | |
Redundancy | 3.4 | |
CC(1/2) | 0.994 | 0.761 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 15 % PEG 2000, 0.1 M HEPES |