8HCT

Crystal structure of Cu2+ binding to Dendrorhynchus zhejiangensis ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	I 4 3 2
Unit cell lengths	151.011, 151.011, 151.011
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	106.780 - 2.260
R-factor	0.16689
Rwork	0.164
R-free	0.22639
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.779
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	BALBES
Refinement software	REFMAC (ccp4-7.0.024)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.900	2.970
High resolution limit [Å]	1.380	1.380
Rmerge	0.065
Rmeas	0.069
Rpim	0.022
Number of reflections	13361	68702
<I/σ(I)>	5.3
Completeness [%]	80.7
Redundancy	7.6
CC(1/2)	0.997	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M sodium citrate tribasic dihydrate, 0.1 M Tris hydrochloride pH 8.5, 30% v/v polyethyene glycol 400