8HCS

zebrafish IRF-11 DBD

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	298.15
Detector technology	CCD
Collection date	2020-10-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 43 3 2
Unit cell lengths	101.324, 101.324, 101.324
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.570 - 2.750
R-factor	0.237
Rwork	0.233
R-free	0.27410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2irf
RMSD bond length	0.009
RMSD bond angle	1.225
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.570	2.650
High resolution limit [Å]	2.550	2.550
Number of reflections	8495	303
<I/σ(I)>	36.4
Completeness [%]	90.2
Redundancy	2
CC(1/2)	1.000	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	1.26 M sodium phosphate monobasic, 0.14 M potassium phosphate dibasic, pH 5.6