8HCL
zebrafish IRF-10 DBD complex with DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 298.15 |
| Detector technology | CCD |
| Collection date | 2020-10-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.010, 86.470, 57.150 |
| Unit cell angles | 90.00, 103.97, 90.00 |
Refinement procedure
| Resolution | 38.830 - 1.990 |
| R-factor | 0.2254 |
| Rwork | 0.223 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2irf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.508 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.19.2_4158) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.460 | 8.900 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 25670 | 1894 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 98.8 | 99 |
| Redundancy | 3.4 | 2.3 |
| CC(1/2) | 0.996 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.1M sodium acetate trihydrate pH 4.5, 25% polyethylene glycol 3350 |
