8HCG
Crystal structure of mTREX1-dAMP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-08-03 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.99984 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 92.585, 92.585, 67.440 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.643 - 1.799 |
R-factor | 0.1669 |
Rwork | 0.165 |
R-free | 0.19160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yws |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
High resolution limit [Å] | 1.799 | 3.880 | 1.800 |
Rmerge | 0.071 | 0.048 | 0.487 |
Rmeas | 0.076 | 0.052 | 0.521 |
Rpim | 0.025 | 0.018 | 0.182 |
Number of reflections | 31351 | 3274 | 3081 |
<I/σ(I)> | 11 | ||
Completeness [%] | 99.9 | 99.5 | 99.9 |
Redundancy | 9.2 | 8.4 | 7.8 |
CC(1/2) | 0.998 | 0.866 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.5, 40% v/v Polyethylene glycol 400 |