8HCE

Crystal structure of mTREX1-CMP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	64.041, 85.655, 100.034
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.077 - 1.501
R-factor	0.1595
Rwork	0.157
R-free	0.18990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yws
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.041	0.022	0.476
Rmeas	0.045	0.024	0.541
Rpim	0.020	0.011	0.249
Total number of observations	386279
Number of reflections	85211	4134	4184
<I/σ(I)>	16.9
Completeness [%]	96.2	87.3	95.6
Redundancy	4.5	4.8	4.2
CC(1/2)		0.999	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium sulfate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350