8HC0

Crystal structure of the extracellular domains of GPR110

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	134.825, 47.993, 87.023
Unit cell angles	90.00, 127.35, 90.00

Refinement procedure

Resolution	19.800 - 2.900
R-factor	0.2355
Rwork	0.233
R-free	0.29180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	predicted by AlphaFold2
RMSD bond length	0.005
RMSD bond angle	0.579
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (phenix-1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.800	43.800	3.070
High resolution limit [Å]	2.900	8.540	2.900
Rmerge	0.124	0.049	0.579
Rmeas	0.149	0.060	0.701
Number of reflections	18390	409	1555
<I/σ(I)>	9.68
Completeness [%]	98.0
Redundancy	3.18
CC(1/2)	0.989	0.995	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	0.2 M ammonium sulfate, 0.1 M sodium acetate trihydrate pH 4.6, 30% PEG-MME 2,000, 15% glycerol