8HBT
AmAT7-3 mutant A310G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 136.020, 136.020, 192.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.530 - 1.960 |
| R-factor | 0.2073 |
| Rwork | 0.206 |
| R-free | 0.22960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8h8i |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.862 |
| Data reduction software | HKL-2000 (HKL-2000) |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHENIX (1.16_3549) |
| Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.530 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.113 | |
| Number of reflections | 49037 | 3426 |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 1.6 M magnesium sulfate, 100 Mm MES/sodium hydroxide pH 6.5 |
