8HBR

The C-terminal domain of Spiral2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97864
Spacegroup name	P 1 21 1
Unit cell lengths	55.509, 109.560, 69.787
Unit cell angles	90.00, 113.20, 90.00

Refinement procedure

Resolution	40.000 - 2.200
R-factor	0.2046
Rwork	0.202
R-free	0.25620
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.620
Data reduction software	XDS
Data scaling software	pointless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.250	46.250	2.230
High resolution limit [Å]	2.160	8.910	2.160
Rmerge	0.066	0.044	0.656
Rmeas	0.072	0.048	0.747
Rpim	0.027	0.018	0.347
Total number of observations	261108	3770	13291
Number of reflections	40398	600	3042
<I/σ(I)>	20.6	59.2	2.3
Completeness [%]	98.1	98.9	85
Redundancy	6.5	6.3	4.4
CC(1/2)	0.999	0.999	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	ammonium sulphate, BICINE (pH9)