8HBR
The C-terminal domain of Spiral2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-28 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97864 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.509, 109.560, 69.787 |
Unit cell angles | 90.00, 113.20, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.200 |
R-factor | 0.2046 |
Rwork | 0.202 |
R-free | 0.25620 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.620 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.250 | 46.250 | 2.230 |
High resolution limit [Å] | 2.160 | 8.910 | 2.160 |
Rmerge | 0.066 | 0.044 | 0.656 |
Rmeas | 0.072 | 0.048 | 0.747 |
Rpim | 0.027 | 0.018 | 0.347 |
Total number of observations | 261108 | 3770 | 13291 |
Number of reflections | 40398 | 600 | 3042 |
<I/σ(I)> | 20.6 | 59.2 | 2.3 |
Completeness [%] | 98.1 | 98.9 | 85 |
Redundancy | 6.5 | 6.3 | 4.4 |
CC(1/2) | 0.999 | 0.999 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | ammonium sulphate, BICINE (pH9) |