8HBC
Crystal structure of the CysR-CTLD3 fragment of human DEC205
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.963, 75.874, 89.595 |
| Unit cell angles | 64.84, 70.07, 84.18 |
Refinement procedure
| Resolution | 29.874 - 3.350 |
| R-factor | 0.2068 |
| Rwork | 0.202 |
| R-free | 0.25380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB:5XTW CTLD1-2 of CD206 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.741 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.880 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rpim | 0.067 | 0.159 |
| Number of reflections | 22317 | 2176 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.2 M Sodium Citrate, 20% PEG3350, final pH 7.4 |
