8HAZ
Crystal structure of Caenorhabditis elegans NMAD-1 in complex with ligand I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2021-10-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.221, 48.528, 51.878 |
| Unit cell angles | 90.00, 104.43, 90.00 |
Refinement procedure
| Resolution | 37.540 - 2.660 |
| R-factor | 0.2504 |
| Rwork | 0.249 |
| R-free | 0.27310 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.989 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 6106 | 597 |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 90.3 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.965 | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 25% PEG 3350, 200 mM, CH3COONH4, 100 mM Bis-Tris pH 6.5 |
