8HAQ
The complex of Src with GW8510
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2022-09-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97914 |
Spacegroup name | P 1 |
Unit cell lengths | 41.830, 62.580, 74.260 |
Unit cell angles | 79.30, 87.98, 89.73 |
Refinement procedure
Resolution | 32.960 - 2.270 |
R-factor | 0.2095 |
Rwork | 0.207 |
R-free | 0.24990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bdf |
RMSD bond length | 0.011 |
RMSD bond angle | 1.288 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.200 | 2.330 |
High resolution limit [Å] | 2.270 | 2.270 |
Rpim | 0.131 | 0.644 |
Number of reflections | 33316 | 2502 |
<I/σ(I)> | 4.4 | |
Completeness [%] | 97.5 | |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MOPS pH 7.0, 18% PEG 3350, 5% Glycerol, 5 mM TCEP |