8H8N
Crystal structure of apo-R52Y/E56Y/R59Y/E63Y-rHLFr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2022-03-01 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.877, 181.877, 181.877 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.852 - 1.500 |
| Rwork | 0.160 |
| R-free | 0.17800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dat |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.962 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.852 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 41696 | 2076 |
| <I/σ(I)> | 30.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10 | |
| CC(1/2) | 1.000 | 0.957 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium sulphate, Cadmium sulphate |
