8H8H

Crystal structure of the N-terminal domain of H-NS family protein TurB (TurB_nt50)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2018-06-09
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	45.692, 63.303, 275.872
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.920 - 2.700
R-factor	0.2505
Rwork	0.246
R-free	0.28510
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.074
Data reduction software	XDS (Jan 10, 2022)
Data scaling software	Aimless (CCP4Interface 8.0)
Phasing software	CRANK2 (CCP4Interface 7.0)
Refinement software	PHENIX ((1.20.1_4487))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.640	3.180	2.830
High resolution limit [Å]	2.700	3.000	2.700
Rmerge	0.140	0.471	1.355
Rmeas	0.146	0.481
Rpim	0.041	0.095	0.375
Number of reflections	22936	2447	2231
<I/σ(I)>	10.9	5.5	2.4
Completeness [%]	98.9	92.4	98.75
Redundancy	13.1		13.8
CC(1/2)		0.996	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	279	PEG 3350, HEPES, ammonium acetate
1	VAPOR DIFFUSION, HANGING DROP	7.6	279	PEG 3350, HEPES, ammonium acetate