8H85
Trans-3/4-proline-hydroxylase H11 with 3-hydroxyl-proline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 75.390, 75.390, 143.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.820 - 2.380 |
| R-factor | 0.1654 |
| Rwork | 0.164 |
| R-free | 0.18810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6LNS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.654 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.420 |
| High resolution limit [Å] | 2.380 | 6.460 | 2.380 |
| Rmerge | 0.067 | 0.032 | 0.502 |
| Rmeas | 0.070 | 0.034 | 0.521 |
| Rpim | 0.020 | 0.011 | 0.141 |
| Total number of observations | 217057 | ||
| Number of reflections | 17231 | 987 | 856 |
| <I/σ(I)> | 8.2 | ||
| Completeness [%] | 99.9 | 98.8 | 100 |
| Redundancy | 12.6 | 10.6 | 13.2 |
| CC(1/2) | 0.999 | 0.969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.15 | 0.7M Sodium citrate tribasic dihydrate and 0.1M Bis-Tris propane (pH 7.0) |
