8H81

Trans-3/4-proline-hydroxylase H11 with 4-Hydroxyl-proline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 41 2 2
Unit cell lengths	75.289, 75.289, 144.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.630 - 1.840
R-factor	0.1509
Rwork	0.149
R-free	0.18190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6LNS
RMSD bond length	0.003
RMSD bond angle	0.939
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.290	1.870
High resolution limit [Å]	1.840	1.840
Rmerge	0.058	0.529
Rmeas	0.059	0.540
Rpim	0.012	0.107
Number of reflections	36880	1801
<I/σ(I)>	152.6	7.8
Completeness [%]	99.9	100
Redundancy	24.4	25.1
CC(1/2)	1.000	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289.15	0.7M Sodium citrate tribasic dihydrate and 0.1M Bis-Tris propane (pH 7.0)