8H7Y
Trans-3/4-proline-hydroxylase H11 with AKG and L-proline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 106.437, 106.437, 145.016 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.790 - 2.140 |
R-factor | 0.15547 |
Rwork | 0.152 |
R-free | 0.21603 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6LNS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.268 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.810 | 2.180 |
High resolution limit [Å] | 2.139 | 2.140 |
Rmerge | 0.132 | 0.529 |
Rmeas | 0.135 | 0.538 |
Rpim | 0.025 | 0.100 |
Number of reflections | 44086 | 2262 |
<I/σ(I)> | 8.3 | 1.3 |
Completeness [%] | 94.5 | 99 |
Redundancy | 28.6 | |
CC(1/2) | 0.981 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.15 | 0.7M Sodium citrate tribasic dihydrate and 0.1M Bis-Tris propane (pH 7.0) |