8H6R

LW Domain of Arabidopsis thaliana TFIIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-10
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 61
Unit cell lengths	65.375, 65.375, 52.446
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.990 - 2.600
R-factor	0.2372
Rwork	0.234
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	predict structure
RMSD bond length	0.005
RMSD bond angle	0.597
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.990	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.121
Number of reflections	3963	3963
<I/σ(I)>	9.9
Completeness [%]	99.6
Redundancy	8.2
CC(1/2)	0.998	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Tris, 0.2 M sodium trihydrate, pH 8.5