8H63

Crystal structure of Internalin A from Listeria monocytogenes with nanobody VHH10 bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-23
Detector	DECTRIS PILATUS 12M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	151.581, 52.420, 103.060
Unit cell angles	90.00, 128.65, 90.00

Refinement procedure

Resolution	31.550 - 1.530
R-factor	0.1617
Rwork	0.161
R-free	0.19020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8H62 6JB9
RMSD bond length	0.011
RMSD bond angle	1.676
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.300	1.610
High resolution limit [Å]	1.530	1.530
Rmerge	0.081	0.564
Rpim	0.042	0.294
Number of reflections	91994	12582
<I/σ(I)>	11.4	2.3
Completeness [%]	96.6	91
Redundancy	4.6	4.5
CC(1/2)	0.996	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.085 M HEPES sodium pH 7.0; 8.5% Isopropanol; 20% PEG 4,000