8H5E
Crystal structure of the MgtE TM domain in complex with Ca2+ ions at 2.5 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-17 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.760, 70.280, 103.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.540 - 2.500 |
| R-factor | 0.2353 |
| Rwork | 0.233 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u9l |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.194 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.540 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 17028 | 2665 |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 35.3 | |
| CC(1/2) | 0.995 | 0.574 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | PEG 400, HEPES, NaSCN |
