8H5C

Structure of SARS-CoV-2 Omicron BA.2.75 RBD in complex with human ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	105.769, 105.769, 228.846
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.040 - 2.900
R-factor	0.199
Rwork	0.197
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lzg
RMSD bond length	0.004
RMSD bond angle	0.608
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	28751	2859
<I/σ(I)>	12.1
Completeness [%]	97.0
Redundancy	6
CC(1/2)	0.991	0.633

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.15 M Ammonium sulfate, 0.1 M Sodium HEPES, 20 % w/v PEG 4000