8H55

The structure of zebrafish angiotensinogen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	42.940, 88.760, 118.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.380 - 2.030
R-factor	0.2202
Rwork	0.219
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ant
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.380	2.103
High resolution limit [Å]	2.030	2.030
Number of reflections	57090	5648
<I/σ(I)>	5.43
Completeness [%]	99.4
Redundancy	1.9
CC(1/2)	0.990	0.474

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298.15	0.2M Potassium Sulfate, 20% PGE 3350