8H4G

Blasnase-T13A/M57N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	173
Detector technology	CCD
Collection date	2021-10-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 41 21 2
Unit cell lengths	92.308, 92.308, 233.134
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.950 - 1.810
Rwork	0.196
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c8x
RMSD bond length	0.011
RMSD bond angle	1.122
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (v1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.950	1.840
High resolution limit [Å]	1.810	1.810
Rpim	0.033	0.642
Number of reflections	92541	4498
<I/σ(I)>	12.3
Completeness [%]	99.9
Redundancy	25.8
CC(1/2)	0.998	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	sodium formate