8H4E
Blasnase-T13A/P55N with D-asn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2021-10-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 92.420, 92.420, 232.452 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.060 |
Rwork | 0.196 |
R-free | 0.22060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7c8x |
RMSD bond length | 0.010 |
RMSD bond angle | 1.072 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (v1.16) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.970 | 2.110 |
High resolution limit [Å] | 2.060 | 2.060 |
Rpim | 0.025 | 0.361 |
Number of reflections | 63183 | 4373 |
<I/σ(I)> | 18.3 | |
Completeness [%] | 99.9 | |
Redundancy | 26 | |
CC(1/2) | 0.999 | 0.829 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | sodium formate |