8H4C
Blasnase-T13A/M57P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2021-10-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.716, 91.716, 233.017 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 1.600 |
| Rwork | 0.183 |
| R-free | 0.19290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c8x |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.063 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.16) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.830 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rpim | 0.021 | 0.423 |
| Number of reflections | 123059 | 6442 |
| <I/σ(I)> | 17 | |
| Completeness [%] | 93.5 | |
| Redundancy | 25.6 | |
| CC(1/2) | 0.999 | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | sodium formate |
