8H4B

Blasnase-T13A/M57P with L-asn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	173
Detector technology	CCD
Collection date	2021-11-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 41 21 2
Unit cell lengths	91.544, 91.544, 231.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.640 - 2.000
Rwork	0.186
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c8x
RMSD bond length	0.009
RMSD bond angle	0.972
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (v1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.640	2.050
High resolution limit [Å]	2.000	2.000
Rpim	0.054	0.461
Number of reflections	67648	4474
<I/σ(I)>	10.9
Completeness [%]	100.0
Redundancy	25
CC(1/2)	0.998	0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	sodium formate