8H46

Blasnase-T13A/P55N with L-asn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	173
Detector technology	CCD
Collection date	2021-11-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 41 21 2
Unit cell lengths	92.418, 92.418, 231.413
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.380 - 1.800
Rwork	0.183
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c8x
RMSD bond length	0.007
RMSD bond angle	0.799
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (v1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.860	1.830
High resolution limit [Å]	1.800	1.800
Rpim	0.021	0.383
Number of reflections	93735	4565
<I/σ(I)>	17.9
Completeness [%]	100.0
Redundancy	24.9
CC(1/2)	0.999	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	sodium formate