8H3I

Crystal Structure of the apo-form Pathogenesis-related Protein HcPR10 from Halostachys caspica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	80
Detector technology	CCD
Collection date	2021-06-04
Detector	RAYONIX MX300-HS
Wavelength(s)	0.97930
Spacegroup name	P 32 2 1
Unit cell lengths	59.426, 59.426, 92.023
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.460 - 1.900
R-factor	0.2013
Rwork	0.199
R-free	0.24780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bv1
RMSD bond length	0.011
RMSD bond angle	1.097
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.089	0.660
Number of reflections	15377	742
<I/σ(I)>	25.8
Completeness [%]	100.0
Redundancy	19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M HEPES sodium 7.5;1.4M Sodium citrate tribasic dihydrate