8H3C

Crystal structure of M2e Influenza peptide in complex with antibody scFv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	104.818, 120.398, 176.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.200 - 3.430
R-factor	0.2056
Rwork	0.204
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dsi
RMSD bond length	0.003
RMSD bond angle	0.619
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.200	3.480
High resolution limit [Å]	3.420	3.420
Number of reflections	30668	1527
<I/σ(I)>	6.7	2.1
Completeness [%]	99.7
Redundancy	4
CC(1/2)	0.983	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.2M Sodium Citrate Dibasic pH 5.2, 20% PEG 1000