8H2U
X-ray Structure of photosystem I-LHCI super complex from Chlamydomonas reinhardtii.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-07 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 194.570, 98.120, 210.130 |
| Unit cell angles | 90.00, 94.56, 90.00 |
Refinement procedure
| Resolution | 47.740 - 3.400 |
| Rwork | 0.291 |
| R-free | 0.35900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xk8 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.740 | 3.320 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.145 | 2.299 |
| Number of reflections | 123685 | 9090 |
| <I/σ(I)> | 11.98 | |
| Completeness [%] | 98.9 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.970 | 0.552 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 50 mM Tris-HCl (pH 7.0), 50 mM Li2SO4 and 4.5-7.0 % (w/v) polyethylene glycol (PEG) 6,000 |
