8H2N
gp96 RNA polymerase from P23-45 phage (crystal 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.158, 128.910, 136.758 |
| Unit cell angles | 90.00, 96.84, 90.00 |
Refinement procedure
| Resolution | 44.370 - 4.410 |
| R-factor | 0.2498 |
| Rwork | 0.245 |
| R-free | 0.30270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8h2m |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.370 | 4.520 |
| High resolution limit [Å] | 4.410 | 4.410 |
| Number of reflections | 50313 | 3691 |
| <I/σ(I)> | 4.08 | |
| Completeness [%] | 99.5 | 98.4 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.989 | 0.191 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 22.5% 2-Methyl-2,4-pentanediol, 12.5% PEG 3350, 100 mM HEPES (pH 7.5), 100 mM NH4OAc, 100 mM MgCl2 |
