8H2M

gp96 RNA polymerase from P23-45 phage (crystal 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978
Spacegroup name	P 41 2 2
Unit cell lengths	137.780, 137.780, 135.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.960 - 3.080
R-factor	0.2091
Rwork	0.205
R-free	0.25380
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.960	3.270
High resolution limit [Å]	3.080	3.080
Number of reflections	46152	7612
<I/σ(I)>	14.7
Completeness [%]	100.0	100
Redundancy	86.9	76.5
CC(1/2)	0.998	0.475

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 1000, 100 mM Tris-HCl buffer (pH 9.0)