8H26
Crystal structure of MnmM from S. aureus complexed with SAH (1.50 A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-29 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.329, 69.362, 74.608 |
| Unit cell angles | 91.25, 112.97, 114.05 |
Refinement procedure
| Resolution | 67.090 - 1.500 |
| R-factor | 0.18451 |
| Rwork | 0.183 |
| R-free | 0.21855 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold model (UniProt: Q2FXG9) |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.652 |
| Data reduction software | XDS (Jan 10, 2022) |
| Data scaling software | STARANISO |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.090 | 1.680 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.069 | 1.285 |
| Rmeas | 0.074 | 1.382 |
| Rpim | 0.028 | 0.507 |
| Number of reflections | 104221 | 5203 |
| <I/σ(I)> | 14.7 | 1.7 |
| Completeness [%] | 92.7 | 72.3 |
| Redundancy | 7.3 | 7.4 |
| CC(1/2) | 0.999 | 0.637 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M BIS-TRIS pH 6.5 and 25% w/v PEG3350 |
