8H25
Lacticaseibacillus casei GH35 beta-galactosidase LBCZ_0230
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.384, 110.998, 120.337 |
Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
Resolution | 45.900 - 2.295 |
Rwork | 0.195 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e8d |
RMSD bond length | 0.003 |
RMSD bond angle | 0.886 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.900 | 2.430 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.085 | |
Number of reflections | 108220 | 17244 |
<I/σ(I)> | 13.24 | 2.38 |
Completeness [%] | 99.7 | |
Redundancy | 3.79 | |
CC(1/2) | 0.998 | 0.768 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES-NaOH buffer (pH 7.5), 10% (v/v) 2-propanol, and 200 g/L polyethylene glycol 4000 |