8H25

Lacticaseibacillus casei GH35 beta-galactosidase LBCZ_0230

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	92.384, 110.998, 120.337
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	45.900 - 2.295
Rwork	0.195
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4e8d
RMSD bond length	0.003
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.900	2.430
High resolution limit [Å]	2.290	2.290
Rmerge	0.085
Number of reflections	108220	17244
<I/σ(I)>	13.24	2.38
Completeness [%]	99.7
Redundancy	3.79
CC(1/2)	0.998	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES-NaOH buffer (pH 7.5), 10% (v/v) 2-propanol, and 200 g/L polyethylene glycol 4000