8H24

Leucine-rich alpha-2-glycoprotein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 63 2 2
Unit cell lengths	143.018, 143.018, 113.728
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.270 - 2.450
R-factor	0.1885
Rwork	0.186
R-free	0.21940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NGL3 3zyn
RMSD bond length	0.009
RMSD bond angle	1.204
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.15.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.450	5.280	2.450
Rmerge	0.175	0.075	0.790
Rmeas	0.177	0.076	0.800
Rpim	0.028	0.013	0.127
Total number of observations	1025968
Number of reflections	25623	2765	2503
<I/σ(I)>	4.4
Completeness [%]	99.9	99.4	100
Redundancy	40	37.4	39
CC(1/2)		0.999	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291	200mM lithium sulfate, 100mM sodium acetate pH4.5, 48% PEG 400 (v/v)