8H21

Serine Palmitoyltransferase from Sphingobacterium multivorum complexed with L-alanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-04-14
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	61.234, 61.234, 208.182
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.956 - 1.540
Rwork	0.172
R-free	0.21640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a2b
RMSD bond length	0.012
RMSD bond angle	1.769
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.540	1.540
Number of reflections	58899	8495
<I/σ(I)>	27.1
Completeness [%]	98.3
Redundancy	17.8
CC(1/2)	0.999	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG4000, sodium acetate, Tris-HCl