8H1W

Serine Palmitoyltransferase from Sphingobacterium multivorum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-11
Detector	RAYONIX MX225-HS
Wavelength(s)	0.9
Spacegroup name	P 41 21 2
Unit cell lengths	61.612, 61.612, 208.349
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.604 - 1.400
Rwork	0.169
R-free	0.20170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a2b
RMSD bond length	0.013
RMSD bond angle	1.895
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.480
High resolution limit [Å]	1.400	1.400
Number of reflections	80227	12715
<I/σ(I)>	20.6
Completeness [%]	100.0
Redundancy	10.8
CC(1/2)	1.000	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	PEG4000, sodium acetate, Tris-HCl