Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.848, 140.475, 133.180 |
| Unit cell angles | 90.00, 104.39, 90.00 |
Refinement procedure
| Resolution | 43.250 - 2.980 |
| R-factor | 0.2355 |
| Rwork | 0.234 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iva |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.271 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.250 | 3.090 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Number of reflections | 66447 | 6661 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 97.3 | |
| Redundancy | 5 | |
| CC(1/2) | 0.920 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | 175 mM citric acid/Bis-Tris (pH 5.0), 17% (v/v) PEG3350 and 3% methanol (as additive) |
