8H1M
Crystal structure of glucose-2-epimerase mutant_D254A from Runella slithyformis Runsl_4512
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPRING-8 |
Beamline | BL45XU |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-10-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 113.219, 113.219, 116.284 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.040 - 1.600 |
R-factor | 0.1451 |
Rwork | 0.144 |
R-free | 0.16620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.525 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.040 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.083 | |
Number of reflections | 99920 | 9806 |
<I/σ(I)> | 20.83 | |
Completeness [%] | 99.9 | |
Redundancy | 13.1 | |
CC(1/2) | 0.999 | 0.890 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 3.6M sodium formate |