8H1K
Crystal structure of glucose-2-epimerase from Runella slithyformis Runsl_4512
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-06-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 113.366, 113.366, 116.031 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.000 - 1.600 |
R-factor | 0.1428 |
Rwork | 0.142 |
R-free | 0.16160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vw5 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.570 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.150 | 0.871 |
Number of reflections | 99680 | 9841 |
<I/σ(I)> | 14.15 | |
Completeness [%] | 100.0 | |
Redundancy | 13.2 | |
CC(1/2) | 0.997 | 0.923 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 3.4 M sodium formate, 20 mM glucose |