8H1I

Crystal structure of PlyGRCS, a bacteriophage Endolysin in complex with Cold shock protein C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97373
Spacegroup name	P 21 21 21
Unit cell lengths	50.667, 55.290, 99.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.650 - 2.100
R-factor	0.1881
Rwork	0.185
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4csh
RMSD bond length	0.007
RMSD bond angle	0.950
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.290	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.137	0.533
Number of reflections	16495	1318
<I/σ(I)>	7.4
Completeness [%]	98.0
Redundancy	4.2
CC(1/2)	0.970	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM MES, pH 6.0, 5 mM CaCl2, 35% PEGMME 5K